Week 2 – Description of Designing Primers and Trainings
Hello everyone,
As described in the previous blog post, the hypothesis was that the cytoplasmic portion of Cxcr3 was important in interacting with downstream components for cell migratory signaling in TNBC. My goal was to create a Cxcr3 mutant with a c-terminal deletion. I designed primers for a PCR process to clone the mutant gene. To design these primers, I found the gene sequence in the NCBI database and wrote out the complementary bases of 23 nucleotides for the start and stop codons around the target gene. I will go into further detail on the PCR process in my next blog post.
This week I mainly completed trainings required for lab access. These included: Preventing Harassment and Discrimination, HIPAA Annual Certification, Bloodborne Pathogens and Universal Precautions (OSHA Compliant), General Laboratory Safety, Basic Biosafety Protection Course, and Fire Safety Awareness. These trainings included the proper procedures for handling lab equipment and responding to accidents involving the equipment. I also scheduled a “Lab Safety Orientation” class where I will be getting familiar with the equipment at the site placement to start hands-on work in the following weeks.
I look forward to keeping these weekly updates as I begin work in the lab soon. Feel free to ask any questions in the comments.
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