Week 2: Advancing KnoxFog™ – Prototype Development and Application Optimization

Shreyash P -

Welcome back to my research blog!

This past week has been full of exciting developments as I continue understanding the KnoxFog™ coating for its application in medical devices, particularly in endoscopic surgery. While I have been working on datat analysis, I want to catch you all up on:

  • Enhancing the durability of KnoxFog™ for prolonged surgical procedures
  • Developing a prototype silicone compartment for controlled application
  • Testing sterilization methods to ensure clinical compatibility

Improving KnoxFog™ Longevity and Stability

In my initial testing, KnoxFog™ demonstrated excellent anti-fogging performance, but prolonged exposure to surgical conditions showed some degradation over time. This week, I worked on modifying the formulation to improve its longevity while maintaining its hyper-hydrophilic properties.

Two key ideas of this new solution is:

  1. It is Semi-Solid – this means that the application is easy to apply since it is still viscous and it takes less time to dry (since it is close to a solid). Other solutions that are purely liquid take too long to dry – potentially increasing surgery time,cost, and scarring – and other solutions that are solid are hard toapply to the lens
  2. It is Hyper-hydrophilic – this means the the solution matches its surface energy with that of water in order to completely flatten the droplet and prevent distortion (shown below in the diagram); increasing surface energy and hydroaffinity will flatten the drople more. Since like attracts like and the solution has the same surface energy as water, the water is compltely flattened on the lens, preventing distortion and increased visibility.

In last weeks blog post, I also included a diagram that shows a 2D representation of hydrophobic vs. hydrophilic vs super and hyper-hydrophilic surfaces. Refer back to last week for a detailed representation.

a) Key Point: Surface energy for super and hyper-hydropilic coatings are the same HOWEVER semi-solid hyper-hydrophilic coatings absorb water way better and the coating lasts longer. Greater the surface energy and hydroaffinity will allow the water droplet to flatten even more

Early results:

✔️ Improved retention – The formulation adhered better to surgical optics, reducing the frequency of reapplications.
✔️ Sustained clarity – Fog-free performance extended beyond 60 minutes in simulated conditions, a major improvement over previous trials.
✔️ Increased chemical resilience – The updated formulation withstood exposure to standard surgical disinfectants without breaking down.

Developing the Silicone Casing Prototype for Precise Application

A key area of focus has been developing a silicone casing that houses and dispenses KnoxFog™. This will ensure controlled and sterile application, reducing contamination risks and making the application process more efficient.

As lead engineer for R&D of the original prototype, the key parts of the prototype development process include:

1. We first create 3 sets of 5 identical mock-up prototypes

  • Each set test a specific, FDA-approbed adhesive OR silicone tape to test adhesion of various polymers

2. Design comprises of two BOXES serving as two compartments

  1. to apply FogKnox™solution 
  2. to DRY the applied FogKnox™  film in five seconds

3. FDA-Grade Polymer Materials USP Class IV Polypropylene outer boxes FDA-Grade  Silicone dabber to apply FogKnox™

Three Adhesive Tested:

  1. Weldbond Glue
  2. Silicone Glue
  3. FDA-Grade SIlicone Tape
b) original casing prototype dimensions and modeling…now known as ClearEndoscope – applicator device of KnoxFog

Since our solution is now semi-solid, we no longer need a drying comparment → this allows for a single compartment silcone casing rather than double.

Whats Coming Up?

Next week, I will focus on scaling up production for more extensive testing, including:

  • Comparative trials with current anti-fogging methods (e.g., heated lenses, alcohol-based coatings, no coating) under varying conditions.
  • The actual experimentaion procedure and design
  • Collaboration with surgeons at Banner Health to gain insights into real-world needs.

In future weeks, we will talk about the results and the comparison of the antifog solution to an uncoated lens, sterility testing, and animal testing.

I’m excited to see how these developments will shape the future of fog-free medical optics. Stay tuned for next week’s updates, where I’ll dive deeper into real-world testing and clinical validation.

As always, feel free to share your thoughts or questions – I’d love to hear your feedback!

Also, I know the difference between the FogKnox vs KnoxFog is very confusing, please let me know if I should make an additional blog post dedicated to clarifying the differences between the two?

Finally, thank you so much to Professor Herbots for providing me with the resources to conduct this research, the mentoring on the science behind this project, and the previous years of experience/foundation to build my project off of. Also, thank you Mr. Schaffer for mentoring me from school on my expectaions and pacing for my project alongside other great expertise.

Best regards and happy readings,
Shreyash

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Comments:

All viewpoints are welcome but profane, threatening, disrespectful, or harassing comments will not be tolerated and are subject to moderation up to, and including, full deletion.

    Viraj Amin
    This is great work that can solve a critical problem that we currently face in the medical field. Keep up the great work Shreyash, and I can’t wait to see your future progress on this project!
    Prof. Nicole Herbots
    Looking good, Shreyash - Great plan!
    Prof. Nicole Herbots
    Looking good, Shreyash - Great plan!
    Vraj Shah
    Shreyash, what an amazing and innovative idea you have come up with. I can’t wait to see where this product goes in the future!
    Abel Dawit
    Nice Work Shreyash! I'm excited to see how well the semi-solid formula works in actual testing because it sounds like a game-changer!
    Abhiram Kilaru
    This is an amazing project you were gifted an opportunity to participate in loved how you went about this.
    Anshul Baddi
    Hey Shreyash, I really like this idea of KnoxFog. I was wondering how do KnoxFog™’s improved retention and clarity reduce surgical interruptions?
    Arya Bhakta
    Great job on this weeks post Shreyash! The science behind how KnoxFogTM works is very compelling. Are there any trade-offs when trying to maximize hyper-hydrophilicity in the coating while also preserving other important lens properties? I can't wait to read your next blog about how KnoxFogTM compares to existing methods of anti-fogging.
    Krish Bhakta
    Awesome work Shreyash! Im so excited to see the applications of your senior project integral to the future of medicine. Through shadowing doctors, I have seen how big of a deal fogging is and I can see how useful this research will be in reducing surgery duration. Keep up the great work!
    Uzayr Ansari
    Wow Shreyash, Trying to find a solution to such a minuscule everyday issue that most people wouldn’t give a second thought about is impressive and the exact mentality which the STEM field needs. What would you say caused you to take it upon your hands to find an answer to this issue rather than to wipe off your lens and look the other way to this problem?
    shreyash_p
    Thank you, Anshul, for your question. The key point is that there's an inverse relationship between Time-To-Fog and overall surgery duration. By increasing the fog-free period on endoscopic lenses, our latest coating reduces the need for surgeons to repeatedly remove and re-insert the lens. Each removal and insertion introduces the risk of infection or scarring and adds extra steps (wiping the lens off, cleaning surrounding areas, reapplying the coating, and accurately reinserting the lens) while maintaining sterility. Therefore, longer fog-free times (which KnoxFog provides) lead to fewer interruptions, which improves both surgical efficiency and patient safety.
    shreyash_p
    Hey Arya, it’s actually really interesting you picked up on that balance between maximizing hyper-hydrophilicity and maintaining other lens properties - especially considering we’ve moved to a semi-solid coating. One challenge we’ve seen with fully liquid coatings is that they can be wiped off too easily during surgery or even breed bacteria if trapped in certain pockets. On the other hand, a completely solid coating might be too difficult to remove when you actually want to replace it. By making KnoxFog™ semi-solid, we’re aiming for the sweet spot: 1. Strong Enough to Stay Put – It adheres well to the lens, so it won’t just rinse off with bodily fluids or standard cleaning during the procedure. This minimizes the need for constant reapplication mid-surgery. Flexible Enough to Remove – Because it’s not fully cured into a hard layer, surgeons can deliberately remove it when needed (for a fresh coat or for sterilization), without having to scrape or damage the lens. 2. Antibacterial Considerations – We actually did do testing for wheter the solution prevents the growth of E-Coli, Lacto Bacillus, and other bacteria. I will elaborate more in future weeks about the process for those but we did find that the hyper-hydrophilic solution did prevent bacteria growth due to one of the chemicals (cannot be mentioned due to NDA) in the composition of KnoxFog. It’s definitely a balancing act, but so far, the semi-solid approach has shown promise in delivering both a robust anti-fog effect and a hygienic, easily manageable coating. Thanks once again!
    shreyash_p
    Overall, thank you to everyone who read my blog and special thanks to anyone who ask questions or left their thoughts on my post. I know alot of my responses are wordy but I am hoping that its easier to understand in layman's terms. Please give me feedback as well as to things I should consider in both my blog posts and reponses to comments. Thank you to everyone once again!
    shreyash_p
    Good question Uzayr! I think being at the forefront of both R&D but also commercialization is a great experience and one I really wanted to be a part of. I saw the implications - from reducing surgical interruptions and potential complications to the broader applications in everyday items (initially from glasses fogging). It’s not just about developing a cool scientific solution in the lab; it’s also about making sure it can truly benefit people in real-world settings. That dual challenge of innovating and translating a product into the market is what excites me most and keeps me motivated.
    Brendan Bang
    This was a really insightful update! I find the semi-solid formulation of KnoxFog™ particularly interesting—especially how it balances ease of application and drying time. How do you think it compares in durability to traditional anti-fog coatings used in endoscopic surgery? Also, would love a post clarifying the difference between FogKnox and KnoxFog! Looking forward to next week’s results!
    Shefali Prakash
    Great work Shreyash! Really impressed by the depth and detail of your blog posts - it's cool that you're able to simplify such complex topics into layman's terms! Looking forward to learning more!
    shreyash_p
    Thank you Brendan for your question regaurding the comparison of KnoxFog to other solutions. Currently, antifog solutions like Clearify or FRED are commonly used in surgeries but last for less than an hour beofre fogging or residue buildup occurs. KnoxFog is able to provide clear visivility for over 1.5 hours and even 2 in some tests!

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