SRP update #8

Jonathan C -

This week, I was able to get a lot done in regards to my research. Last Friday, I completed treating atria with Phenylephrine (PE), simply put a chemical used to induce hypertension within the atrial tissues. We then homogenized these atria with the PE and then we froze the samples. On monday, we took the frozen samples and performed what’s called a Bradford assay, that is to say a colorimetric time-tested assay. This sounds fancy, but it is actually a relatively simple process to determine the amount of protein within a sample. We do this to set our experiment up for the western blotting that I will be performing on Monday-Tuesday back to back next week. This is a longer process, so it must be done on back to back days. Due to the simplicity of my project, these couple weeks are all I needed in order to complete my experiment. Any questions are always welcome, and I am excited to share my findings during my presentation later this month. 

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    caitlin_e
    Hi Jonathan! From the curriculum you were working on earlier to the new research duties you have now, it has been very intriguing to see your project take shape. I'm looking forward to seeing your results in the final presentation! What are your predictions for the western blotting test that you are conducting next week?
    April 4, 2025 at 2:08 pm - Reply
    camille_bennett
    Hi Jonathan, great insight into your lab work. What has it been like getting into the lab?
    April 10, 2025 at 8:39 am - Reply
    jonathan_c
    Hi Ms. Bennett, thank you for the question. Getting into the lab was a relatively easy process, however it was outside my expectations. To some degree, I expected some sort of training and/or paperwork, but there was much more planning and paperwork involved than I had originally thought. It was a bit tedious, but definitely was not hard to get through.
    April 20, 2025 at 10:59 pm - Reply
    jonathan_c
    Hi Caitlin, thank you for your question. I am sending this response a bit in the future, but I can tell you what I had hoped for and what I ended up with. I was hoping for some nicely visible bands of clipped ANP from the Western Blot. Contrary to this belief, let alone clipped ANP, there wasn't any full length ANP bands either in my ex-vivo samples. I believe this error to be due to incomplete denaturing of the proteins, and if given more time I would have liked to run another Western Blot. However, all I can say for right now is that my research is unfortunately inconclusive. Hope this answers your question!
    April 20, 2025 at 11:01 pm - Reply

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