Charlotte B's Senior Project Blog

Project Title: Bee-licious: Do Honeybees Select Protein Rich Pollen Based on Their Seasonal Nutrient Needs?
BASIS Advisor: Kristen Sanders
Internship Location: Carl Hayden Bee Research Center
Onsite Mentor: Dr. Vanessa Corby-Harris



Project Abstract

Nearly one hundred kinds of crops require pollination by honeybees! Honeybees provide us with just under one third of the food we eat, as they are the main pollinators of large-scale crop monocultures such as almond, avocado, potatoes, fruits, and coffee. However, in recent years honeybee colonies have experienced drastic population declines due to a variety of environmental threats including climate change, new pathogens, and pesticide usage. In order to protect honeybees, we must conduct research to better understand their health and nutritional requirements. A balanced diet of pollen and nectar is necessary for honeybees to form healthy colonies capable of withstanding environmental stressors. More precise components of pollen, such as proteins are especially important for honeybees’ growth, development, and reproduction. Honeybees rely on protein to create jelly that feeds developing larvae. In late winter and early spring colonies grow by producing more brood, but in the fall, colonies produce less brood and begin preparing for overwintering. Do honeybees have the ability to differentiate between pollens and collect higher protein pollens according to their seasonal nutrient needs? I hope to answer this question at Carl Hayden Bee Research Center by measuring the protein concentration of pollen collected from each season of a year. Understanding how honeybees change their pollen collection process throughout seasonal cycles will support broader implications about their ability to adapt and survive in the face of ongoing environmental stressors.

    My Posts:

  • Week 11: The Beeginning of the End

    Hi everyone! This week got off to a slow start, as I was stuck at home with a bad case of strep throat. Luckily though there were lots of tasks I could work on here at the house. After lots of cutting, glueing, and pasting I finally finished making my research poster! I'm really happy... Read More

  • Week 10: ‘Pollen’ it All Together!

    Hi everyone! This week I spent hours putting together my PowerPoint presentation! So far, I'm surprised by how long my PowerPoint has ended up being (25 slides). I know the presentation is only supposed to be about ten minutes, so I think I might have to make some cuts. The biggest challenge I had while... Read More

  • Week 9: The Beesults Are In!

    Hi everyone! This week I finally finished calculating the protein concentrations for all of my samples. Yay! My mentor looked through all of my data tables and everything seemed good, however she did notice something about my June and July samples. In my previous blogs I wrote about how I had to dilute the June... Read More

  • Week 8: Buzzing Through Dilutions and Data

    Hi everyone! This week I returned from my little trip to WashU. Admitted Students Day was a lot fun! I got to explore campus, hangout with other students, and eat some delicious food. I'm definitely excited to go back in August! I returned to the lab on Monday and started the week off by making... Read More

  • Week 7: I’ve Been a Busy Bee!

    Hi everyone! It has been a very busy week in the lab with a variety of different tasks! On Monday I started by finishing my last plate which included my December and January samples. I was very excited to finish the data collection part of my project (or so I thought) .... When my mentor... Read More

  • Week 6: Beeyond Blue!

    Hi all! I had a very busy week in the lab and nearly finished all of my data collection! I started the week by preparing new standard solutions for the protein assay. My mentor told me it's important to make new standard solutions each week even if I still have some leftover from last week... Read More

  • Week 5: Spring Break!

    Hi everyone! I had an awesome week working as a camp counselor at the JCC. We took the kids on so many field trips including the zoo, Dave & Busters, Bens Bells, and the park! It was a lot of fun, but I am definitely exhausted and ready to return to the lab on Monday.... Read More

  • Week 4: Plates Full of Pollen!

    Hi everyone! I spent a lot of time in the lab this week! I mentioned in my last blog that I learned how to prepare a plate for the protein assay, but when the spectrophotometer read the plate some of my samples were out of range. This meant I would need to dilute the samples... Read More

  • Week 3: This Pipette is Bee-coming my Best Friend

    Hi everyone! I've had a very productive week at the lab! On Tuesday I started by transferring 1350 microliters of pollen into each of the tubes I labeled last week. We only had a pipette that can hold up to 1000 microliters, but since I needed 1350 microliters, I had to pipette 675 microliters of... Read More

  • Week 2: Bee Organized!

    Hi everyone! I've had a very busy week at the lab! My first task was to search through the freezer and find all the pollen samples that I'll be using for my research. The samples were collected in 2021 and represent 15 different honeybee colonies. Additionally, my samples come from two different yard sites called... Read More

  • Week 1: What’s the Buzz about Safety and Security?

    Hi everyone! The past few months I've been working on obtaining government clearance to work in the USDA lab. I thought this would be as simple as filling out a few forms. Boy was I wrong! The whole process began back in October when I submitted the first round of paperwork.  Next, in January I... Read More

  • Introduction

    Hello everyone! My name is Charlotte Balla, and I am a senior at BASIS Tucson North. For my senior research project, I'll be working in a USDA lab to analyze pollen protein content and determine if honeybees collect pollen based on their seasonal nutrient needs. You're probably wondering what made me want to study honeybees?... Read More