David Guo Week 4 Update
David G -
Dear readers,
This week, I continued my experiments where I started counting my colonies and also photographed my plates. Last week I was able to inoculate my plates and also start the incubation process for the plates. This took 48 hours, and after that, I was able to see colonies being formed on the plates. Specifically, these colonies are bacteria, and each colony looks like a little dot. On the plates, I started photographing. I noticed on the first dilution that there were too many colonies to count. This is because there were too many bacteria in the sample that we used, which created many colonies. However, going down the dilution ladder, when I finally got to 10 to the -9, there were colonies that I was able to count. Compared to the first dilution, there was less bacterial growth, and this is because we diluted the solution by 10 to the -9 following this process. This will help us qualify how much bacteria we had in the original sample. Specifically, by counting how many colonies we had in the 10 to the -9 solution, we can estimate how many colonies there would be in the 1.0 solution. This is extremely important for qualification, and having an accurate experiment. In addition to this, I will be preparing for the viral part of my experiment in the next few weeks.
Besides experiments, I also continued my design of experiments. Because I will be continuing the virus part next week, I worked to refine my design of experiment and prepared samples for those experiments.
I am excited for the outcome of my experiments! Thank you for reading!
Sincerely,
David Guo
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