Week 1: I’m making something!… It will be great!…

Sumin S -

Hello, my name is Sumin! I’m a senior at BASIS San Antonio Shavano Campus planning to pursue a career in the field of materials as well as biomedical engineering! Welcome to my Senior Project blog! For the next 10 weeks, I will post my experiences and the things I learned throughout this journey of Senior Project. I am extremely happy and grateful to get this opportunity to work under the guidance of Dr. Gongchen Sun, assistant professor at the University of Texas at San Antonio in the Department of Biomedical and Chemical Engineering. I will be working closely with his graduate student Gabriel G who is in the 2nd year of the Ph.D. program. I’m planning to go in for about 4 hours a day every Monday, Tuesday, and Thursday, and for the rest of the week, I will spend my time reviewing the literature to get a more in-depth understanding of the biomedical engineering field since it is a new branch of engineering for me.

 On my first day at the lab, which was this Monday, Gabriel gave me a quick tour of the lab and general information on the research that he is currently working on with Dr. Sun. He explained to me that the goal of this project is to develop a new mechanism and technique to separate the cells. One of the fundamental challenges in the field of biomedical engineering or any other fields that require cell samples is the inability to extract and maintain the cell sample fresh without any damage or contamination; in fact, almost 80% of the extracted cell samples are either damaged or contaminated. This challenge was one the main reasons that led Dr. Sun to study cell separators and design one that can extract cell samples effectively.

Even though Gabriel described every single detail of the research step-by-step, it was still difficult for me to fully understand all the magical scientific words (especially with two solutions named PEG and DEX). But, in my understanding, this research is using the two-phase system, meaning two different types of solution that don’t mix, to separate fresh living cells from dead damaged cells. The two solutions, PEG and DEX, form distinct layers due to their different chemical properties. When a sample containing cells is introduced into this system and subjected to centrifugation force, the living, and dead cells distribute themselves between the two layers based on their surface properties and densities. Fresh, living cells tend to migrate into one phase, while dead or damaged cells remain in the other, enabling efficient separation.

3D Model of Two-Phase System
3D Model of Two-Phase System

My task in this project is to design and develop a centrifugation spinner that could capture an instant result. According to Dr. Sun, one of the major problems in the research was not being able to view the sample immediately under a microscope right after the sample was centrifuged. The tiny movement that results from the transport of the sample from the centrifugation spinner to the microscope caused the sample to be compromised. For instance, an air bubble formation can make it difficult to obtain good results. To assist in solving this difficulty, my Senior Project will be designing a laboratory spinner that has a microscope camera.

Connecting two pins with a wire
Connecting two pins with a wire

During my first two days in the lab, I focused on deconstructing the spinner provided to me and exploring ways to bypass its built-in sensor. In order to capture real-time results of the sample, the spinner needed to continue operating even when the lid was open. However, the safety mechanism in place automatically stopped the spinning motion once the lid was lifted. To overcome this challenge, I had to disable the sensor entirely. My approach involved circulating the electrical current within the spinner, allowing it to function without interruption. Of course, I did not arrive at this solution on my own. With Gabriel’s guidance, I was able to identify the necessary modifications. For the next few weeks, I will keep studying more about the spinner itself and make a design of this spinner.

That is all for this week! Thank you so much for your time and I hope you enjoyed reading my first post!

More Posts

Comments:

All viewpoints are welcome but profane, threatening, disrespectful, or harassing comments will not be tolerated and are subject to moderation up to, and including, full deletion.

    srisnigdha
    Hi Sumin, your project sounds challenging! The process of figuring out how to modify the spinner and bypass the sensor sounds tricky, but it’s awesome that you’re learning so much in the process. What got you interested in the field of materials? Could you explain a little more about what it is? I have never really learned anything about it before. Best of luck with your design, and I look forward to seeing how it evolves!
    david_t
    Hello Sumin! Your project seems very interesting and challenging, but it sounds like your project will help you dive into your field and gain valuable experience. Could you explain a little bit about your thought process from when you found the problem of the safety mechanism to when you implemented your solution? I look forward to seeing how your design progresses!
    sumin_s
    Hey Snigdha! Thank you so much for reading my blog! I was first introduced to the field of material from a curiosity of aluminum foil. As I begin to research and learn more about the aluminum foil itself, I truly fall in love with the diversity and complexity within this field! The material engineering can be pretty much anything; so, asking the question "what is this made of?" will be the fundamental topic of this field. Thank you!
    sumin_s
    Hey David! I'm glad that you enjoyed my blog! On my first day at the site, my on-site advisors provided me with a centrifugation spinner that had a transparent lid. However, to take a clearer picture, we decided to remove the lid; this was mostly because we could not clean the lid clearly and the microscope would catch every dust :(. So, our solution to this was to take off the lid. As it explained in my blog, there was a safety mechanism implemented into the spinner, and the only way to bypass that was to turn off the sensor while making the spinner to keep moving. The only way to do this way giving a pathway that the current can circulate thoroughly. I hope this answers your question!
    jack_s
    Hey Sumin! The issue of disturbances arising in the solution from simply carrying the sample over to the microscope is tricky! It's one of those things that you don't really think about but it totally makes sense that it could cause issues when examining. You mention adding a microscope camera to the centrifuge, is there any reason you would choose this over a lens that a researcher would look through? Your project seems very interesting and I am excited to see where it ends up!
    sumin_s
    Hey Jack! Thank you for reading my blog, and I'm glad that you enjoyed it! To answer your question, the main reason why I used a microscope camera over a lens was purely due to the size of the sample that we are currently examining. It was a little too small to be observed from the lens. But I think putting a lens on the lid is a great idea! I might refer to that when I design the spinner! Thank you!

Leave a Reply

Your email address will not be published. Required fields are marked *