Week 8: Scaling and FFTs

Shaima U -

Hi all, it’s been quite a week since I got the stir bars last time. I’ve progressed in the sense that I’ve collected some data, but I’ve been trying to scale down the reaction even more. The first couple trials of this have been quite inconsistent. I haven’t been getting successive oscillations, the blue starch-triiodide complex has been really pale, and at times, I run out of one solution before the others (there should be an equal ratio of all solutions…). I originally started off my journey by making a total solution (A+B+C) of 30ml , but I tried scaling it down by a factor of 5 to a total of 6ml. This is important because I don’t want to use too much of my deuterated materials aimlessly in one go, so the less materials I can use, the better. All hope isn’t lost, however. Last week, I scaled the reaction down by half, and I was able to get pretty consistent results! I created a Fast Fourier Transform (FFT) which gives insight into what frequencies are most apparent throughout each plots’ oscillations: 

 

FFT of BR scaled to 15ml
FFT of BR scaled to 15ml

We can see a great peak between 0.15-0.17 Hz. I’ll be using this plot again when I collect data from my deuterated trials. I can’t say I know what will happen, though, because I can make an argument in the case that there is a slight or great change in frequency. I don’t expect absolutely no change, but we’ll see what happens when I get there (hopefully by later this week!).

Separately, I’ve also been working on a method for analysis based partly on theory. A previous study on the sibling reaction of BR, the Belosouv Zhabotinsky (BZ) reaction, has been done under deuterated acidic conditions as well. In the paper, they had developed a means to find the rate constant, k1, of their enolization reaction through theory given their specific experimental conditions. Through this, they were able to find that the enolization reaction actually demonstrated a primary KIE as opposed to the secondary KIE that extraneous research has demonstrated in the enolization reaction under similar acidic conditions (under sulfuric acid). Essentially, I’m hoping to proof this method of analysis by conducting it under the BR reaction, and we’ll see what happens from there!

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Comments:

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    Anonymous
    Hi Shaima, What does the peak in your graph mean for your experiment?
    Anonymous
    I'm not sure why this says its anonymous but it's Maggie Krause
    magdalena_k
    Just to make sure I get credit for commenting I'm just going to ask the question again. What does the peak in your graph mean for your experiment?
    inika_b
    Hi Shaima! So what's your plan should the method of analysis that you're attempting fails?
    shaima_u
    Hi Maggie! The peaks in the graph represent the most apparent frequency throughout the course of the full oscillations. Sometimes I'll get a not-so-obvious peak where the peak sort of spreads out. This would represent a gradual change in the frequencies of oscillations.
    shaima_u
    Hi Inika! I'm interpreting "fails" as finding unreasonable differences between our results. If this occurs, then I would suggest that the method of analysis is either not suited to understand the reactions because of some other underlying complexity in either or both reactions. In this case, I would need to consider more elementary steps for the kinetic analysis. Hope this answered your question!

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